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Mccance And Huether Pathophysiology 8th Mendeley Pdf

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Methods: For in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for in vivo studies. Enzyme activity was determined spectrophotometrically by the Beutler method. Results: The in vitro study showed that the I 50 value was 17 mM for ethanol. Conclusions: The results of this study suggest that ethanol has a significant inhibitory effect on the G6PD activity both in vivo and in vitro. The most common red blood cell enzyme defect throughout the world is glucose 6-phosphate dehydrogenase G6PD deficiency Weksler et al.

Pathophysiology Apa Format

Methods: For in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for in vivo studies. Enzyme activity was determined spectrophotometrically by the Beutler method. Results: The in vitro study showed that the I 50 value was 17 mM for ethanol. Conclusions: The results of this study suggest that ethanol has a significant inhibitory effect on the G6PD activity both in vivo and in vitro.

The most common red blood cell enzyme defect throughout the world is glucose 6-phosphate dehydrogenase G6PD deficiency Weksler et al. G6PD deficiency is an X chromosome-linked trait, fully expressed in males and homozygous females and is variably expressed in heterozygous females Aksoy et al.

Sometimes, G6PD deficiency disorder is also referred to as primaquine sensitivity or favism Weksler et al. Some drugs primaquine, aspirin, sulphonamids etc. A major role of NADPH in erythrocytes is regeneration of reduced glutathione, which prevents haemoglobin denaturation, preserves the integrity of red blood cell membrane sulphydryl groups, and detoxifies hydrogen peroxide and oxygen radicals in and on the red blood cells Deutsch, ; Weksler et al.

Ethanol is a widely consumed sedative—hypnotic drug throughout the world Lee and Becker, It has been shown that ethanol intake may lead to oxidative damage in several tissues such as brain, stomach, liver or erythrocyte Bondy and Guo, ; Sozmen et al. Ethanol increases the generation of reactive oxygen species in these tissues and its acute intake decreases reduced glutathione levels in plasma and erythrocytes Loguercio et al. Since ethanol has oxidant effects in erythrocytes, it was considered important to reveal the effect of ethanol consumption on erythrocyte G6PD activity, which has not been studied before.

Therefore the objective of this study was to investigate the effect of ethanol on G6PD enzyme activity in vitro in human, and in vivo in rat, erythrocytes. All other chemicals used were analytical grade and were purchased from either Sigma or Merck, Germany. Preparation of the haemolysates. Fresh human blood collected in tubes with EDTA 5 mM was centrifuged at g for 15 min and the plasma and leucocyte coat were removed by aspiration. The packed red cells were washed with 0.

Ammonium sulphate fractionation and dialysis. Ammonium sulphate was slowly added to complete dissolution. The mixture was centrifuged at g for 15 min and the precipitate was dissolved in 50 mM phosphate buffer pH 7. Preparation of affinity gels. The gel was washed with distilled water to remove foreign bodies and air in the swollen gel was eliminated. The gel was suspended in 0. The gel was washed with equilibration buffer. Purification of G6PD by affinity chromatography. Washing continued up to an absorbance of 0.

Eluates were collected in 2 ml tubes and the activity of each was separately calculated. Active fractions were collected. Protein determination. Quantitative protein determination was performed spectrophotometrically at nm by the method of Bradford , with bovine serum albumin as standard.

Sodium dodecyl sulphate SDS —polyacryamide gel electrophoresis. This was performed after the purification of enzyme by the method of Laemmli Ethanol was used as the inhibitor.

Activities were measured at 0. Ten adult male Sprague—Dawley rats with a weight of — g were used for the experiment. The animals were housed individually and were fed with standard laboratory chow and water before the experiment. Twenty-four hours before the experiments, the rats were starved, but were allowed access to water ad libitum.

For control measurements, a 0. At 1, 3 and 6 h after ethanol administration, 0. All blood samples were added to EDTA tubes. Haemolysates were prepared as described for the in vitro studies. G6PD was purified fold with a yield of SDS—polyacrylamide gel electrophoresis was performed after purification of the enzyme and the electrophoretic pattern obtained is shown in Fig. Our study revealed that ethanol inhibited the G6PD activity of rat and human red blood cells in in vivo and in vitro conditions, respectively.

The I 50 value obtained from in vitro studies was 0. The data from the in vivo studies Table 2 using rat erythrocytes show that ethanol significantly inhibited the activity of G6PD.

The ethanol contents of various beverages varies between 2. Together with some beneficial effects, ethanol exerts serious side-effects in the CNS, liver and other tissues Rang et al. It is known that ethanol increases the generation of reactive oxygen species and leads to oxidative damage in erythrocytes Sozmen et al. In fact, ethanol causes several haemolytic disorders due to both direct and indirect effects Lee and Becker, Additionally, the ethanol metabolite acetaldehyde inside of the erythrocyte has the ability to generate free radical species and to cause deleterious effects on erythrocytes Tyulina et al.

After ethanol intake, its blood level rises to a peak within 30—90 min Laurence et al. The half-life of ethanol in plasma is highly variable, but a single dose of ethanol will not all be disposed of for 6—8 h or even more Laurence et al.

In our study, maximal inhibition of G6PD activity was within 1 h after ethanol administration, and inhibition continued significantly after 3 h Table 2. These results suggest a correlation between plasma peak level of ethanol and maximal inhibition of G6PD enzyme activity, and between the plasma half-life of ethanol and continuity of inhibition.

Moreover, the results of our study show that the ethanol itself is a highly potent inhibitor of erythrocyte G6PD enzyme activity in vitro. Based on our results, we believe that the inhibitory effect of ethanol on G6PD enzyme activity at least may be one of the causes of ethanol-induced haemolysis. In conclusion, the present study suggests that patients with G6PD deficiency should avoid ethanol intake.

If they consume any ethanol, they should not be administered oxidant drugs such as analgesics and antipyretic, which frequently inhibit G6PD activity. If it is required to give these drugs to the patient with G6PD deficiency, their dosage should be strictly determined to minimize the haemolytic side-effects. Effects of various concentrations of ethanol in vitro on purified glucose 6-phosphate dehydrogenase G6PD from human erythrocyte.

Effect of ethanol administration on rat erythrocyte glucose 6-phosphate dehydrogenase activity. Sodium dodecyl sulphate—polyacrylamide gel electrophoresis of glucose 6-phosphate dehydrogenase G6PD purified by affinity gel chromatography. Lanes 1 and human carbonic anhydrase-1; lanes 2—5: buffalo milk lactoperoxidase; lanes 6—9: human G6PD. Aksoy, K. Human Genetics 76 , — Andrews, M.

Mosby-Year Book Inc. Berkow, R. Beutler, E. Academic Press, London. Blood 84 , — Bondy, S. European Journal of Pharmacology , — Bradford, M. Analytical Biochemistry 72 , — Delgado, C. Journal of Chromatography , — Deutsch, J. Hernandez-Munoz, R. Laboratory Investigation 80 , — Kayaalp, S. Laemmli, D. Nature , — Laurence, D. Churchill Livingstone, Singapore. Lee, N. Appleton and Lange, USA.

Lindi, C. Alcohol 16 , — Loguercio, C. Italian Journal of Gastroenterology and Hepatolology 29 , — Morelli, A. Ninfali, P. Preparative Biochemistry 20 , — Rang, H. Churchill Livingstone, China. Shreve, D.

Inflammation

Locate a current medical or scientific news or journal article based on Alterations in Cerebral Hemodynamics As you read through the article, think about how it connects to Pathophysiology and why is it relevant 2 Provide a write-up summarizing the article Within your document, include how the information connects to Pathophysiology, and why it is. Description Stressed about the ins and outs of pathophysiology? Gain confidence, with the newly updated Pathophysiology Made Incredibly Easy! Pathophysiology of Disease An Introduction to Clinical Medicine is a book offering a very comprehensive and completely case-based review of the important concepts of pathophysiology It provides an extensive coverage of all major organs and organs systems of the body thus making available all useful information into a single book. Knowledge Checks and Diagnostic Quizzes - Interactive. Pathophysiology Across The Lifespan Discussion Pathophysiology Across The Lifespan Discussion Disorders of Motor Function John is 63 years old and receives home care by an occupational therapist twice a week His therapist is currently working with John on maintaining joint flexibility and balance.


Porth's Pathophysiology: Concepts of Altered Health States (9th ed.). Philadelphia: Wolters Kluwer. McCance, K.L. & Huether, S.E. (). Understanding (8th ed.). Philadelphia: F.A. Davis Co. Townsend, M.C. & Morgan, K.I. (). Essentials of Facebook Twitter · Share; Printer-friendly version · PDF version · Log In.


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Learn, understand, and master pathophysiology! Corresponding to the chapters in Pathophysiology: The Biologic Basis for Disease in Adults and Children, 8th Edition , this study guide offers practical activities to help you review and remember basic pathophysiology. Cellular Biology 2. Altered Cellular and Tissue Biology 3. Genes and Genetic Diseases 5.

Inflammation was described as early as BC in an Egyptian papyrus [1] and is still a common problem despite continuous advancements in prevention and treatment methods. The delineation of the site and extent of inflammation are crucial to the clinical management of infection and for monitoring the response to therapy [2]. Inflammation was described as early as BC in an Egyptian papyrus [ 1 ] and is still a common problem despite continuous advancements in prevention and treatment methods. The delineation of the site and extent of inflammation are crucial to the clinical management of infection and for monitoring the response to therapy [ 2 ]. This issue is relevant to nuclear medicine, since physiological along with morphological imaging has an important role in achieving this goal.

Reference List

Уже несколько лет Танкадо пытался рассказать миру о ТРАНСТЕКСТЕ, но ему никто не хотел верить. Поэтому он решил уничтожить это чудовище в одиночку. Он до самой смерти боролся за то, во что верил, - за право личности на неприкосновенность частной жизни. Внизу по-прежнему завывала сирена. - Надо вырубить все электроснабжение, и как можно скорее! - потребовала Сьюзан.

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Attuigote 31.05.2021 at 10:26

With easy-to-read , in-depth descriptions of disease, disease etiology, and disease processes, Pathophysiology: The Biologic Basis for Disease in Adults and Children, 7th Edition helps you understand the most important and the most complex pathophysiology concepts.

Cid Y. 02.06.2021 at 22:27

8th Edition. The Biological Basis for Disease in Adults and Children. Authors: Kathryn McCance Sue Huether.

Debra V. 03.06.2021 at 13:38

Pathophysiology - 7th Edition - ISBN: , Written by well-known educators Kathryn McCance and Sue Huether, and joined by a team of expert contributors, this resource is the Unit VIII: The Hematologic System Scopus · ScienceDirect · Mendeley · Evolve · Knovel · Reaxys · ClinicalKey.

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